Autoradiography is an experimental technique in which a radioactive specimen is placed in contact with (or close to) a photographic plate, so as to produce a record of the distribution of radioactivity in the specimen.
Autoradiography is the use of X-ray (or occasionally photographic) film to detect radioactive materials. It produces a permanent record of the positions and relative intensities of radiolabeled bands in a gel or blot.
History
The first autoradiography was obtained accidently around 1867 when a blackening was produced on emulsions of silver chloride and iodide by uranium salts. Such studies and the work of the Curies in 1898 demonstrated autoradiography before, and contributed directly to, the discovery of radioactivity. The development of autoradiography as a biological technique really started to happen after World war II with the development of photographic emulsions and then stripping film (see Rogers, 1979) made of silver halide. Radioactivity is now no longer the property of a few rare elements of minor biological interest (such as radium, thorium or uranium) as now any biological compound can be labelled with radioactive isotopes opening up many possibilities in the study of living systems.
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